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Halo DB 20 / DB 20S Spectrophotometer

 UV / Visible Double Beam Spectrophotometer Halo DB 20 / DB 20S Spectrophotometer

The Halo DB-20 is a high performance double beam spectrophotometer suitable for many analytical applications where a higher level of accuracy is required.

 Spectral Features
Coma aberration elimination from the concave diffraction grating achieves a high resolution 1.5nm (DB-20S : 1nm) spectral band pass and certifies compliance of the Halo DB-20 to the stringent European Pharmacopoeia standards.  Other specifications include an impressive wavelength accuracy of 0.3nm, noise level 0.0003Abs (500nm) and stray light 0.05% (220nm NaI, 340nm NaNO2
 Built-in and Diverse Range of Measurement Modes
 Photometry Mode
Perform quantitative analyses in either absorbance or transmittance modes.  Select from single wavelength, up to 6 different individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves with up to 20 standards for concentration measurements.
 Time Scan
Perform kinetic measurements for time periods ranging from 1 minute to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.
 Wavelength scan
Perform a full spectral scan from 190 to 1,100nm at any of 8 incremental and preset selectable scan speeds starting from a high resolution 10nm/minute up to a swift 3,600nm/minute.  Data is displayed as either numerical values or a graphical spectrum. Furthermore perform downstream processing of data, such as peak/valley search or smoothing, directly on board or with the optional Halo UV Detective software.
 Dual Lamp Advantage
By virtue of the long life, halogen tungsten and deuterium lamps, the Halo DB-20s wavelength range is an extensive 190nm - 1,100nm.  Besides, the dual lamp system results in higher accuracy than corresponding xenon lamps.  Lamp switching is automatic and selectable from a wavelength range of 325nm to 370nm.
 User Friendly Operation and Information Rich LCD Display
The extra-large 190mm x 138mm, backlit LCD screen with adjustable brightness control displays a large array of data also in graphical format.  The seamless and chemical resistant keypad is designed for easy and quick selection of navigation and function features whilst protecting against any laboratory spills.
 Validation Functions
To ensure optimum instrument performance, self-diagnosis incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up.  The Halo DB-20 is also equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, band pass, baseline flatness, baseline stability and noise level can be all validated and the audit report printed.
 Stand alone or PC Operation
The Halo DB-20 is fully equipped and capable of executing all functions in standalone mode. Simply connect a standard laser printer for direct printouts of data and graphs.  For more advanced control, analyses and reporting, the simple slide of a switch places the Halo DB-20 under the direct control of the optional UV Detective software.
 On-Board Data Storage
Up to 20 operating programs and up to 10 sets of measurement data can be stored in the flash memory of the Halo DB-20. Programs can easily be recalled, edited and deleted.  In standalone mode, data (in text format) can be downloaded directly to an external memory stick via the USB port and transferred for further processing to any computer loaded with commercial spreadsheets (such as Microsoft Excel)


Concave diffraction grating / Double Beam Principle

Wavelength Range

190nm -1,100 nm

Spectral Bandwidth

1.5 nm/ 1.0 nm

Stray Light

 ≤0.05% (220nm NaI, 340nm NaNO2)/ ≤0.10% (220nm NaI, 340nm NaNO2)

Wavelength Accuracy


Photometric Range

Absorbance: -3 to +3 %T: 0% to 300%T Concentration: 0,000 to 9,999

Wavelength Scan Speed

10, 100, 200, 400, 800, 1,200, 2,400, 3,600 nm/minute

Wavelength Slew Speed


Baseline Flatness

0.0003 Abs/hr (500nm, after 2 hours)

Baseline Stability

 0.0004 Abs/hr (500nm, after 2 hours)

Noise Level

 0.0003 Abs (500nm)

Light Source

Tungsten-Halogen and Deuterium Lamps

Light Source Switching

Automatic switching selectable from 325nm to 370nm


Silicon Photodiode


Back-lit LCD 165(W) x 122(H) mm


505(W) x 590(D) x 265(H) mm

Net Weight


Gross Weight


Power Requirements

110 - 220 V selectable, 50/60Hz

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